The ban on buses, trains and Luas trams includes e-scooters that can be folded or carried.
It will apply to e-scooters, but not e-bikes or mobility scooters.
When announcing the ban in August, the NTA said it is due to a risk of fire posed by batteries inside some poor quality devices.
The guidance is prompted by safety concerns in relation to lithium-ion batteries which are commonplace in such devices.
The NTA said due to the newness of e-scooters, the quality control of their construction is not as mature or well developed as e-bikes and mobility scooters, which have been regulated for longer.
“The guidance is prompted by safety concerns in relation to many lithium-ion batteries which are commonplace in such devices. These batteries are known to develop internal faults, leading to overheating and combustion,” it said.
Lithium-ion batteries can develop internal faults, leading to what is known as ‘thermal runaway’, whereby the battery becomes overheated leading to higher and higher temperatures and potentially resulting in the emission of toxic fumes, extreme heat, fire and potentially explosion.
Until very recently, there were no technical standards for e-scooters in Ireland and with a wide variety of e-scooters available, or already purchased, they are unlikely to meet the standards required under new regulations, which prompted the ban on public transport.
Similar restrictions are already in place in Berlin, Barcelona, and the UK.
The ban applies to all services operated under a contract with the NTA, including Dublin Bus, Bus Éireann, Go-Ahead Ireland, Iarnród Éireann, Luas and TFI Local Link.
The restriction is subject to periodic review by the NTA and the transport operators and will likely be reviewed as early as next year.
It follows previously introduced restrictions on the use of e-scooters, which were mostly unregulated until this year.
Three people in total have lost their lives on Irish roads this year while using e-scooters, which equals the amount who were killed last year while using the devices.
In May, the use of electric scooters by children under the age of 16 in a public place was prohibited, under regulations signed by Minister for Transport Eamon Ryan.
A 20kph speed limit and a prohibition of seats on e-scooters was also brought in.
